QuantiFERON-TB Gold Plus Test as a parameter of Mycobacterium tuberculosis detection

Amila Abduzaimović, Sabina Mahmutović-Vranić, Velma Rebić, Mufida Aljičević, Kadrija Abduzaimović, Anes Jogunčić


Background: Latent tuberculosis infection (LTBI) is a state of persist immune response to Mycobacterium tuberculosis (M. tuberculosis) antigens without any clinical evidence of active tuberculosis. A new progress and inovation in diagnostics of latent tuberculosis, more specific and susceptible than tuberculin skin test, are Interferon Gamma Release Assay, Quanti FERON-TB test, in vitro blood tests used to detect immune response to TB proteins, by measuring interferon gamma (IFN-γ( in whole blood from persons infected with M. tuberculosis. The aim of this retrospective study was to determinate the role of QuantiFERON-TB Gold Plus (QFT – Plus test) test in diagnostics of LTBI evaluating the results from inpatients and outpatiens.

Methods: This was a retrospective study where blood samples were collected from 1st of January to 31st of December 2016 in the Microbiological Laboratory of General Hospital Tesanj. Samples were performed from inpatients and outpatients in Zenica-Doboj Canton. Full blood from patients aged from 1 to 95 years was collected in four, special QFT plus blood test tubes and tested with QFT – Plus test.

Results: The study involved 159 patients who were tested with QFT-Plus test. Gender distribution of positive data (30.82 %) showed prevalence of males (59.18 %). The most common diagnosis of positive data was infiltratio pulmonum (49 %). Sensitivity of QFT – Plus test was 91.67 % and test showed specificity of 73.79 %.

Conclusion: Our research showed that QFT – Plus test can be helpful, as a supplement, but it can not be the main parameter in diagnostics of tuberculosis. If the test is positive, it is necessary to observe all other clinical findings to complete the diagnosis.

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